Our All-in-One Grow Bags combine everything you need to grow mushrooms in a single, self-contained unit - All you need to do is add your own spores or liquid culture.

Each bag has the following:

• Sterilized grain for initial colonization
  
  
• Dung-loving mushroom substrate (manure-free and coir-based)
  
  
• A self-healing injection port for easy inoculation
  
  
• A 0.2-micron filter patch for gas exchange and contamination protection
  

They’re designed to support start-to-finish mushroom cultivation entirely inside the bag, so no sterile transfers or extra equipment are needed, making them ideal for newer growers.

Our All-in-One for Dung Loving Mushrooms is ideal for growing dung-loving mushrooms, which naturally fruit on manure-rich environments. This includes many species commonly grown in monotubs, shoeboxes, and trays, or mushrooms that grow on manure or compost substrates.

Note: For wood-loving species like lion’s mane or oyster mushrooms, use our Wood-Loving AIO instead.

These bags are designed to be inoculated using a spore syringe or liquid culture syringe via the self-healing injection port. No need to open the bag or use a still air box.

Here is what you'll need:

• All-in-One Grow Bag
• Gloves
• Liquid Culture or Spore Syringe (Not included)
• 70% Isopropyl Alcohol Spray or Wipe
• A torch or lighter to sterilize your syringe needle

Inoculation Steps:

• Put on gloves, spray your bag, syringe, and hands - wipe down and allow to dry.
• Flame-sterilize the needle until red hot, then let it cool for a few seconds.
• Insert the needle through the injection port and inject 3–5 mL of solution into the bag.
• Move the bag to an incubation area that is dark, room temp, and clean. Do not mix or shake.

Incubation is an important part of the process!

Try not to disturb your mushrooms too much, and leave them in an environment with these conditions:

Temperature: 70-78°F

Lighting: Darkness or low light

Environment: Clean area with access to oxygen & fresh air out of direct sunlight

The full process usually takes 4–8 weeks, depending on:

• Whether you use spores or liquid culture
• Incubation temperatures (ideal range is about 68°F or room temperature)
• Species or quality of culture/spores
  

A typical timeline for growing looks like this:

• 0-2 weeks: First signs of white mycelial growth typically about 15-20% colonization by 2 week mark.
• 2-5 weeks: "Break and Shake" - breakup and redistribute mycelium at 2 week mark, then reform block to promote full colonization. Wait until full colonization of white mycelium covers the block.
• 5-8 weeks: Fruiting and harvest

A "break and shake" is a simple way to speed up and promote colonization of mycelium throughout your all-in-one.

Because mycelium tends to colonize the grain near the injection site first, the break and shake helps distribute mycelium and trigger colonization of the substrate.

After about 2 weeks, or 15-20% colonization of the substrate, growth may begin to stall, indicating it is time for a break and shake. Do this process only once during your grow. Once you break and shake, you'll just have to wait out the full colonization for about 2-3 more weeks.

Your bag is ready to fruit when all conditions are met:

• The substrate is completely white
  
• It feels firm and solid
  
• You may see pins or primordia forming
  
• There are no signs of mold or contamination
  
  

To Initiate Fruiting:

1. Cut a 1-2" slit beneath the filter patch when you see small mushrooms forming.
2. Thats it. Seriously.
3. Seriously, why are you still reading?

Fruiting in the bag is a simple process - the bag will maintain the ideal humidity and airflow needed to support your mushrooms as they grow. The more you mess with it, the more likely you are to introduce contamination to future flushes.

It's generally recommended to maintain room temperature during fruiting. Your Grow Bag should maintain the ideal conditions for growth as the moisture from the substrate and bag conditions provide the ideal humidity for fruiting.

If you are growing in an especially dry area, or your mushroom bag appears dry without condensation on the sides, you may do a light spray with distilled water once per day through the slit in the bag.

Typically you can expect 2-3 flushes per mushroom block.

• First flush is the most productive
  
• Second and third flushes are smaller
  
• Total yield varies by species and environment
  

For Second and Third Flushes:

• Let the block rest for 4–5 days
  
• Spray inside of bag
• Close bag and monitor for growth over 2 week period.

Not for indoor cultivation—but it’s still useful!

• Add to compost piles or garden beds
  
• Use as mulch or soil amendment
  
• Inoculate outdoor mushroom beds
  
  

Troubleshooting slow colonization:

Contamination – green, black, or pink spots; foul smell are indicators of contamination. This can come from improper technique or from sub-par inoculum.

Temps too low/high – below 65°F or above 80°F can slow or stall growth

Overhydration – water pooling in the bag

Old or weak spawn/cultures – expired or improperly stored inoculum may not perform as intended

If unsure, contact Redwood Mushroom Supply with photos for assistance.

An easy way to verify the integrity of your culture or spores is to add a few drops of liquid to an agar plate in a still air box. It should show signs of white mycelial growth in a few days. If you see blotchy, slime like growth, vibrant colored molds, or anything that doesn't look like mycelium on your plate, you likely have a bad culture.

Doing this check before injecting is a good way of saving yourself the frustration and money associated with failure, so we always recommend it!