Pioppino Mushroom Liquid Culture Syringe

Pioppino (Cyclocybe aegerita)

The Pioppino Mushroom is a lesser-known delight among gourmet mushroom growers. Despite being rarely available in markets, the Pioppino is prized for its ease of growth, rich nutty flavor, and its uniquely firm texture. Commonly found on hardwood trees in cooler climates, it cultivates exceptionally well. This species produces generous clusters of small to midsize fruit composed of velvety brown caps with crisp stems.

Our Pioppino strain has frequently impressed, and is one of the fastest to go when grown here at RMS - it is a staff favorite, and continues to impress! We recommend top fruiting this strain, but starting in a bag is certainly recommended until small fruits appear, at which point opening the bag will allow for maximum airflow and help minimize stretching of the stems. Adding rubber bands to minimize side pinning is recommended, as with most top-fruiting mushrooms. Expect a full canopy of delicious velvety caps when growing this strain!

About Our Cultures

All RMS Liquid Cultures are prepared from early-generation slants and plates from our culture bank. These cultures are then expanded into liquid culture using lab-quality inputs, ensuring a vigorous culture ready for further expansion or immediate use. 

Each culture is hand packaged in a clean-air laboratory environment, batch tracked, and quality assured to ensure your culture is contaminant-free and ready to use. If you ever have any issues with our cultures or any questions, feel free to contact us and we can put you in touch with our in-house mycologist.

Our liquid cultures can be injected directly into a wood-based substrate bag with injection port, or into grain bags/jars for expansion and spawn-to-bulk transfers. 

NOTE: Wood-loving species can be added to wood substrates without the need for a grain transfer - to get more mileage out of your culture, inoculate grain and add to your substrates in a still air box or flow hood at a  5-10% grain to substrate ratio. 

Liquid Culture Instructions

• PREPARE YOUR ENVIRONMENT:  Wash your hands and put on a pair of disposable gloves. Use a laminar flow hood or a still air box for best results. Thoroughly clean your workspace and gloves with 70% isopropyl alcohol. 
• DISINFECT PACKAGING: Spray syringe and needle packaging thoroughly with 70% isopropyl alcohol. 
• ASSEMBLE SYRINGE: Remove the cap from the liquid culture syringe and attach the needle, twisting to lock it in place. Leave the guard on the needle until you’re ready to inoculate. 
• DISINFECT GRAIN JARS OR BAGS: Disinfect items to be inoculated with 70% isopropyl alcohol.
• INOCULATE: Carefully remove the needle guard and inject the culture. For best results, use a new needle for each injection or flame sterilize between uses. 
• For smaller grain jars, use 3-5mL of liquid culture per jar. 
• For larger bags of grain or wood substrate (3 lbs+) use 10mL of liquid culture per bag.