Pioppino Plate Culture

Pioppino (Cyclocybe aegerita)

The Pioppino Mushroom is a lesser-known delight among gourmet mushroom growers. Despite being rarely available in markets, the Pioppino is prized for its ease of growth, rich nutty flavor, and uniquely firm texture. Commonly found on hardwood trees in cooler climates, it cultivates exceptionally well. This species produces generous clusters of small to midsize fruit with velvety brown caps and crisp stems.

Our Pioppino strain has frequently impressed and is one of the fastest to go when grown here at RMS – it’s truly a staff favorite. We recommend top-fruiting this strain from a bag. Expect a full canopy of delicious velvety caps when growing this strain!

The Pioppino mushroom is versatile in the kitchen, adding a rich, nutty flavor and a firm, crunchy texture to a variety of dishes. It pairs well with meats, vegetables, and grains, making it a favorite among chefs and home cooks alike. Additionally, the longer shelf life of Pioppino mushrooms makes them a practical choice for both fresh consumption and storage.

About Our Cultures

All RMS Plate Cultures are prepared from early-generation slants from our culture bank. These robust cultures are used to inoculate agar plates prepared with high-quality inputs, ensuring you receive vigorous and reliable cultures. 

Each culture is hand-packaged in a clean-air laboratory environment. Our cultures are batch-tracked and undergo thorough quality control to ensure robust growth and contamination-free conditions. If you ever have any questions or issues, feel free to contact us for expert mycology advice.

Our plate cultures provide a robust starting point for mushroom cultivation. For optimal results, we recommend transferring agar from the colonized petri dish to sterilized grain bags or jars. Once fully colonized, the grain spawn can be used to inoculate wood-based substrate at a 5-10% grain-to-substrate ratio. Perform transfers using a still-air box or laminar flow hood to increase the success of your culture expansion.

Plate cultures can also be used to create liquid cultures for bulk inoculation and can be made into additional plates or slants for storage and future use. 

Storage

For best results, store your plate culture in the refrigerator until ready to use. Ensure the plate is fully sealed before storing. If you plan to use the culture within 1-3 weeks, refrigeration is not necessary. When refrigerated, plate cultures can remain viable for up to 6 months. Do not freeze, as this can damage the mycelium. Keep out of direct sunlight and avoid exposure to heat.

Plate Culture Use Instructions

EQUIPMENT CHECKLIST

• RMS Plate Culture
• Sterilized grain
• Disposable gloves
• 70% isopropyl alcohol in a spray bottle
• Scalpel
• Tool sterilizer or open flame for sterilizing tools
• Sealing film
• Recommended: Laminar flow hood or still-air box
  

INSTRUCTIONS

1. PREPARE YOUR ENVIRONMENT

• Wash your hands and put on a pair of disposable gloves. 
• Disinfect your workspace and gloves with 70% isopropyl alcohol. 
• For best results, use a laminar flow hood or a still-air box when working with open cultures. 

Note: Maintaining a clean environment is essential for successful inoculation and optimal culture growth.

2. DISINFECT MATERIALS

• Remove plate culture from the filter bag.
• Spray the exterior of the sealed plate culture and items to be inoculated with 70% isopropyl alcohol. 
• Allow alcohol to evaporate completely before inoculating.

Note: Always use sterile tools when working with cultures. Use a tool sterilizer to sterilize tools between transfers. If using an open flame to sterilize your tools, exercise extreme caution while using isopropyl alcohol in the same area.

3. INOCULATE

• Remove the sealing film and petri dish lid from plate culture. 
• Using sterile tools, carefully cut a 1 cm × 1 cm wedge of agar from the plate culture and transfer it to sterile grain, agar, or liquid culture. Replace petri dish lid when complete and seal your inoculated bag, plate or jar. 
• Now is a great chance to transfer your culture to additional sterile agar plates to create backup cultures.

4. COLONIZE

• Place the inoculated bag, plate, or jar in a 70-75° F environment to encourage mycelial growth.