This bag is designed to support both in-bag growing and 'side fruiting' of gourmet mushrooms like Oyster, Lion's Mane, Chestnut, and Pioppino.

Using a liquid culture syringe containing gourmet mushroom mycelium is recommended with this product. Simply inject the liquid culture of your choice and watch it grow.

Each bag has the following:

• Sterilized grain for initial colonization
  
  
• Fast Fruiting Mix mushroom substrate - a blend of soy hull and oak sawdust
  
  
• A self-healing injection port for easy inoculation
  
  
• A 0.2-micron filter patch for gas exchange and contamination protection
  

These bags are designed for wood-loving species that thrive on enriched sawdust substrates, including:

Oyster Mushrooms, Lion's Mane, Chestnut Mushrooms, and Pioppino.

We recommend pairing these species with this kit as they are the most compatible and will give the best results.

These bags are designed to be inoculated using a liquid culture syringe via the self-healing injection port. No need to open the bag or use a still air box.

Here is what you'll need:

• All-in-One Grow Bag
• Gloves
• Liquid Culture Syringe (Not included)
• 70% Isopropyl Alcohol Spray or Wipe
• A torch or lighter to sterilize your syringe needle

Inoculation Steps:

• Put on gloves, spray your bag, syringe, and hands - wipe down and allow to dry.
• Flame-sterilize the needle until red hot, then let it cool for a few seconds.
• Insert the needle through the injection port and inject 3–5 mL of solution into the bag.
• Move the bag to an incubation area that is dark, room temp, and clean. Do not mix or shake.

Incubation is an important part of the process!

Try not to disturb your mushrooms too much, and leave them in an environment with these conditions:

Temperature: 70-78°F

Lighting: Darkness or low light

Environment: Clean area with access to oxygen & fresh air out of direct sunlight

The full process usually takes 4–8 weeks, depending on:

• Quality of liquid culture
• Incubation temperatures (ideal range is about 68°F or room temperature)
• Species (Oyster and Lion's mane are faster, Chestnut and Pioppino can be slower)
  

A typical timeline for growing looks like this:

• 0-2 weeks: First signs of white mycelial growth typically about 15-20% colonization by 2 week mark.
• 2-5 weeks: "Break and Shake" - breakup and redistribute mycelium at 2 week mark, then reform block to promote full colonization. Wait until full colonization of white mycelium covers the block.
• 5-8 weeks: Fruiting and harvest

A "break and shake" is a simple way to speed up and promote colonization of mycelium throughout your all-in-one.

Because mycelium tends to colonize the grain near the injection site first, the break and shake helps distribute mycelium and trigger colonization of the substrate.

After about 2 weeks, or 15-20% colonization of the substrate, growth may begin to stall, indicating it is time for a break and shake. Do this process only once during your grow. Once you break and shake, you'll just have to wait out the full colonization for about 2-3 more weeks.

Your bag is ready to fruit when all conditions are met:

• The substrate is completely white
  
• It feels firm and solid
  
• You may see pins or primordia forming
  
• There are no signs of mold or contamination
  
  

To Initiate Fruiting:

1. Cut a 2-3" slit or "X" into the side or front of the bag.
2. Place in a location with good ventilation, indirect light, and high humidity. A kitchen counter is a good location.
3. Mist the cut area and continue to mist 2-3 times daily until small mushrooms begin to form.
4. Mist once per day after mushrooms begin to grow up until harvest.

Note: If you are in a drier climate, mist up to 3 times daily if your mushrooms appear dry.

Can't babysit your mushrooms? Grow Tents can be easily automated to meet the ideal conditions for any mushroom without the need for regular misting.

If you're using a Grow Tent:

Maintain 80-95% humidity depending on the species you are growing, and maintain airflow for best results.

Typically you can expect 2-3 flushes per mushroom block.

• First flush is the most productive
  
• Second and third flushes are smaller
  
• Total yield varies by species and environment
  

For Second and Third Flushes:

• Let the block rest for 4–5 days
  
• Spray inside of bag
• Close bag and monitor for growth over 2 week period.

Not for indoor cultivation—but it’s still useful!

• Add to compost piles or garden beds
  
• Use as mulch or soil amendment
  
• Inoculate outdoor mushroom beds
  
  

Troubleshooting slow colonization:

Contamination – green, black, or pink spots; foul smell are indicators of contamination.

Temps too low/high – below 65°F or above 80°F can slow or stall growth

Overhydration – water pooling in the bag

Old or weak spawn/cultures – expired or improperly stored inoculum may not perform as intended

If unsure, contact Redwood Mushroom Supply with photos for assistance.